Purification and Properties of S-Adenosyl-L-methionine: L-Methionine S-Methyltransferase from Wollastonia biflora Leaves
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منابع مشابه
Purification of rat liver S-adenosyl-L-methionine decarboxylase.
The amount of S-adenosyl-l-methionine decarboxylase present in rat liver was enhanced 17-fold by administration of methylglyoxal bis(guanylhydrazone),* a specific inhibitor of the enzyme. The enzyme was purified 1400-fold in 50% yield from such liver extracts by chromatography on columns of the inhibitor bound to Sepharose. The purified enzyme had no spermidine synthetase activity.
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An S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase (SUMT) activity has been identified in Methanobacterium ivanovii and was purified 4,500-fold to homogeneity with a 38% yield. The enzyme had an apparent molecular weight of 58,200 by gel filtration and consisted of two identical subunits of Mr 29,000, as estimated by gel electrophoresis under denaturing conditions. The Km value f...
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The final step in the biosynthesis of mycophenolic acid involves the transfer of a methyl group from S-adenosylmethionine to demethylmycophenolic acid. The enzyme, S-adenosylmethionine:demethylmycophenolic acid O-methyltransferase, was isolated from Penicillium stoloniferum and purified 2,700-fold by ammonium sulfate fractionation and diethylaminoethyl-cellulose and Sephadex G-200 chromatograph...
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Adomet synthetase (EC 2.5.1.6) catalyzes the formation of Adomet, which serves as a methyl group donor in highly specific transmethylation reactions involving a11 kinds of acceptor molecules such as proteins, nucleic acids, phenylpropanoid derivatives, polysaccharides, cyclic fatty acids, etc. Adomet also plays a role in amino acid biosynthesis and polyamine biosynthesis. In plants, Adomet has ...
متن کاملTumor uptake studies of S-adenosyl-L-[methyl-11C]methionine and L-[methyl-11C]methionine.
Tumor accumulation of S-adenosyl-L-[methyl-11C]methionine ([11C]SAM) was investigated in mice bearing mammary carcinoma (FM3A) and in rats bearing ascitic hepatoma (AH109A). After injection of [11C]SAM the blood clearance of 11C radioactivity was rapid. The 11C level was relatively high in both tumors. The uptake ratios of tumor to organ increased with time in several organs, especially in brai...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1995
ISSN: 0021-9258
DOI: 10.1074/jbc.270.38.22344